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Pig Interferon β (IFN-β) ELISA Kit


種屬

Pig (Sus scrofa; Porcine)

UniProt號

N/A

縮寫

IFN-β

別名

IFNB1; IFB; IFF; IFNB; MGC96956; interferon beta

線性范圍

Request Information

靈敏度

Request Information

樣本類型

Serum, Plasma, Other biological fluids

檢測方法

Sandwich

分析方法

Quantitive

反應時間

1-4.5h

樣本體積

1-200 μL

檢測波長

450 nm

貨號

AE64534PI

規格

48T 96T 96T*5 96T*50

價格

查詢

貨期

3-5 working days

說明書

登陸
原理:
This assay employs a two-site sandwich ELISA to quantitate IFN-β in samples. An antibody specific for IFN-β has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-β present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for IFN-β is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of IFN-β bound in the initial step. The color development is stopped and the intensity of the color is measured.

產品概述:
IFN beta elicits a markedly higher antiproliferation response in some cell types such as, embryonal carcinoma, melanoma and melanocytes than do IFN alphas. Higher potency of IFN beta in treatment of multiple sclerosis and certain cancers has been observed. Type I IFNs signal through binding to a common cell surface receptor. Two chains of the receptor, IFNAR1 and IFNAR2, have been identified. Both chains are necessary for function and in the absence of either there is neither high affinity binding nor biological activity. The intracellular portions of the receptor subunits are bound by tyrosine kinases, Jak1 and Tyk2, members of the Janus kinase family. Upon ligand binding these kinases are activated and phosphorylate members of the STAT family of transcription factors, as well as IFNAR1 and 2.

組份:

Reagents

Quantity

Reagents

Quantity

Assay plate (96 Wells)

1

Instruction manual

1

Standard (lyophilized)

2

Sample Diluent

1?x 20 mL

Biotin-Conjugate (concentrate?100 x)

1 x?120?μL

Biotin-Conjugate Diluent

1 x 20?mL

Streptavidin-HRP ?(concentrate?100 x)

1 x 120?μL

Streptavidin-HRP Diluent ??

1 x 20?mL

Wash Buffer (concentrate?25?x)

1 x 20?mL

Substrate Solution

1 x 12?mL

Stop Solution

1 x 10?mL

Adhesive Films

4

特異性:
This assay has high sensitivity and excellent specificity for detection of Pig IFN-β. No significant cross-reactivity or interference between Pig IFN-β and analogues was observed.

回收率:
Matrices listed below were spiked with certain level of recombinant Pig IFN-β and the recovery rates were calculated by comparing the measured value to the expected amount of Pig IFN-β in samples.

Sample Type

Number

Recovery range (%)

Average(%)

Serum

10

90-101

96

EDTA plasma

10

89-97

93

Heparin?plasma

10

91-99

95

精密度:
Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100 Intra-Assay: CV<8% Inter-Assay: CV<12%
線性:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Pig IFN-β and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample Type

1:2

1:4

1:8

1:16

Serum

78-89%

81-99%

92-103%

95-105%

EDTA plasma

91-101%

90-98%

93-101%

91-98%

Heparin?plasma

92-103%

93-102%

92-99%

91-101%

穩定性:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. The loss rate was determined by accelerated thermal degradation test. Keep the kit at 37°C for 4 and 7 days, and compare O.D.values of the kit kept at 37°C with that of at recommended temperature. (referring from China Biological Products Standard, which was calculated by the Arrhenius equation. For ELISA kit, 4 days storage at 37°C can be considered as 6 months at 2 - 8°C, which means 7 days at 37°C equaling 12 months at 2 - 8°C).
樣本收集及儲存:
Serum: Use a serum separator tube (SST) and allow samples to clot for two hours at room temperature or overnight at 2 - 8°C before centrifugation for 15 minutes at 1000 × g. Remove serum and assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Plasma: Collect plasma using EDTA, or heparin as an anticoagulant. Centrifuge for 15 minutes at 1000 × g at 2 - 8°C within 30 minutes of collection. Assay immediately or aliquot and store samples at ≤ -20°C. Avoid repeated freeze-thaw cycles. Other biological fluids: Centrifuge samples for 20 minutes at 1000 × g. Remove particulates and assay immediately or store samples in aliquot at -20°C or -80°C. Avoid repeated freeze/thaw cycles.
注意事項:
Store at 2-8°C. Please refer to Instruction Manual.
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